文章摘要

神经生长因子及其受体在盆腔器官脱垂患者阴道前壁组织中的表达及意义

作者: 1李洁, 1陈飞, 1金玉茜, 1李蕾, 1胡琳, 1占长玉, 1廖营营, 1张莹莹, 1王鲁文
1 郑州大学第三附属医院妇科盆底重建病区,郑州 450052
通讯: 王鲁文 Email: wangluwen631221@163.com
DOI: 10.3978/j.issn.2095-6959.2020.10.007
基金: 河南省医学科技攻关计划普通项目(201602125)。

摘要

目的:探讨神经生长因子(nerve growth factor,NGF)及其低亲和力p75神经营养素受体(p75 neurotrophin receptor,p75NTR)和高亲和力酪氨酸激酶受体A(tyrosine kinase A,TrkA)在盆腔器官脱垂(pelvic organ prolapse,POP)患者阴道前壁组织中的表达及意义。方法:选择30例因阴道前壁膨出行手术治疗的POP患者,根据POP-Q分度法分为两组,其中14例为POP-Q II度,16例为POP-Q III~IV度;同期选择15例非盆底功能障碍性疾病(pelvic floor dysfunction,PFD)患者为对照组。术中取患者阴道前壁相似部位组织,采用RT-PCR和免疫组织化学染色检测NGF,p75NTR和TrkA的表达情况。结果:PCR结果显示对照组患者阴道前壁组织中NGF,p75NTR,TrkA的mRNA表达量分别为1.01±0.06,0.86±0.05,0.95±0.05,POP-Q II度组中为0.47±0.07,0.79±0.05,0.43±0.04,POP-Q III~IV度组中为0.41±0.03,0.53±0.04,0.25±0.19,差异有统计学意义(P<0.05)。且POP组p75NTR/TrkA比值较对照组增大。免疫组织化学染色显示POP患者NGF,p75NTR,TrkA的表达水平均降低。结论:NGF及其受体p75NTR和TrkA在POP患者阴道前壁组织中表达减少以及两受体比例的变化,可能与POP的发生、发展相关。
关键词: 盆腔器官脱垂; 神经生长因子;p75NTR;TrkA

Expression and significance of nerve growth factor and its receptor in anterior vaginal wall of patients with pelvic organ prolapse

Authors: 1LI Jie, 1CHEN Fei, 1JIN Yuxi, 1LI Lei, 1HU Lin, 1ZHAN Changyu, 1LIAO Yingying, 1ZHANG Yingying, 1WANG Luwen
1 Department of Pelvic Floor Reconstruction, Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China

CorrespondingAuthor: WANG Luwen Email: wangluwen631221@163.com

Foundation: This work was supported by Medical Science and Technology Program General Project of Henan Province, China (201602125).

Abstract

Objective: To investigate the expression and significance of nerve growth factor (NGF) and its receptors p75 neurotrophin receptor(p75NTR) and tyrosine kinase A (TrkA) in the anterior vaginal wall of patients with pelvic organ prolapse (POP). Methods: A total of 30 patients with anterior vaginal wall prolapse were selected and divided into two groups according to POP-Q grading method. Among them, 14 cases were POP-Q II degree and 16 cases were POP-Q III–IV degree; 15 non pelvic floor dysfunction (PFD) patients were selected as a control group during the same period. Similar parts of the anterior vaginal wall were taken during the operation. The expressions of NGF, p75NTR and TrkA were detected by RT-PCR and immunohistochemical staining. Results: RT-PCR showed that the mRNA expression levels of NGF, p75NTR and TrkA in the anterior vaginal wall of the control group were 1.01±0.06, 0.86±0.05, 0.95±0.05, in the group of POP-Q II degree were 0.47±0.07, 0.79±0.05, 0.43±0.04 in the group of POP-Q III–IV degree were 0.41±0.03, 0.53±0.04, 0.25±0.19, respectively and the difference was significant (P<0.05). And the ratio of p75NTR/TrkA in the POP group was higher than that in the control group. Immunohistochemical staining showed that the expression levels of NGF, p75NTR and TrkA were decreased in POP patients. Conclusion: The decreased expression of NGF and its receptors p75NTR and TrkA in the anterior vaginal wall of POP patients and the change of the ratio of the two receptors may be related to the occurrence and development of POP.
Keywords: pelvic organ prolapse; nerve growth factor; p75NTR; TrkA