文章摘要

液基细胞学、肿瘤标志物SP70联合检测对肺腺癌转移胸腔积液的诊断价值

作者: 1,2钱同, 2樊昱彤, 3金玉, 3朱立强, 2,3顾兵, 2,3马萍
1 徐州医科大学附属徐州儿童医院检验科,江苏 徐州 221006
2 徐州医科大学医学技术学院,江苏 徐州 221004
3 徐州医科大学附属医院检验科,江苏 徐州 221002
通讯: 樊昱彤 Email: 769046504@qq.com
马萍 Email: pingma62@163.com
DOI: 10.3978/j.issn.2095-6959.2020.10.004

摘要

目的:探讨液基细胞学与肿瘤标志物SP70联合检测对肺腺癌转移胸腔积液的诊断价值。方法:回顾性分析徐州医科大学附属医院2018年11月至2019年3月收治肺腺癌转移胸腔积液27例和非癌性胸腔积液12例,收集放化疗前患者的胸腔积液,采用美国SurePathTM PrepStain全自动液基薄层细胞制片染片机(BD TriPath)及相关耗材制备液基薄片,巴氏染色用于细胞学诊断,同时采用双抗体夹心ELISA法对39份胸腔积液标本上清进行SP70检测。统计分析液基细胞学联合SP70检测对恶性胸腔积液诊断的敏感性、特异性、符合率。结果:以27份癌性胸腔积液和12份非癌性胸腔积液SP70的ELISA检测结果作ROC曲线,分析得出胸腔积液SP70>9.73 ng/mL,可以帮助预测肺腺癌转移胸腔积液,AUC为0.824,采用该截断值诊断肺腺癌转移胸腔积液的敏感性、特异性分别为70.4%和91.7%。液基细胞学与肿瘤标志物SP70联合检测的符合率为89.7%,灵敏度为81.4%,均高于单独液基细胞学的检测,差异有统计学意义(P<0.05)。结论:液基细胞学联合肿瘤标志物SP70有助于提高肺腺癌转移胸腔积液的诊断。
关键词: SP70;液基细胞学;腺癌;肺癌;胸腔积液;诊断

Value of tumor marker SP70 detection combined with liquid based cytology in diagnosing lung adenocarcinoma in pleural effusion

Authors: 1,2QIANG Tong, 2FAN Yutong, 3JIN Yu, 3ZHU Liqiang, 2,3GU Bing, 2,3MA Ping
1 Department of Laboratory Medicine, Xuzhou Children’s Hospital, Xuzhou Medical University, Xuzhou Jiangsu 221006, China
2 Medical Technology School of Xuzhou Medical University, Xuzhou Jiangsu 221004, China
3 Department of Laboratory Medicine, the Affiliated Hospital of Xuzhou Medical University, Xuzhou Jiangsu 221002, China

CorrespondingAuthor: FAN Yutong Email: 769046504@qq.com

Abstract

Objective: To assess the value of tumor marker SP70 level, liquid based cytology examination and combining two methods in predicting lung adenocarcinoma in pleural effusion. Methods: A total of 39 patients in the Affiliated Hospital of Xuzhou Medical University from November 2018 to March 2019 were retrospectively reviewed, including 27 patients with lung adenocarcinoma in pleural effusion and 12 patients with benign pleural effusion. The pleural effusion before radiochemotherapy was collected and detected by liquid-based cytology, meanwhile, SP70 level was detected by the method of ELISA. Sensitive, specificity and accuracy were applied to assess the diagnosis value of two methods in predicting lung adenocarcinoma in pleural effusion. Results: ROC curve for SP70 level in pleural effusion was applied and under curve area was calculated. SP70>9.73 ng/mL helped to predicting lung adenocarcinoma in pleural effusion with the sensitivity of 70.4%, specificity of 91.7%. The area under ROC curve was 0.824. The accuracy and sensitivity of SP70 combined with liquid based cytology were 89.7% and 81.4%, both higher than liquid based cytology alone. The difference was statistically significant (P<0.05). Conclusion: Liquid based cytology and SP70 level were useful in predicting lung adenocarcinoma in pleural effusion to a certain degree. Combining 2 methods could improve the sensitivity and accuracy.
Keywords: SP70; liquid based cytology; adenocarcinoma; lung cancer; pleural effusion; diagnosis