病毒宏基因组学在HPV感染相关性疾病中的研究进展
| 作者: |
1胡慧敏,
1马红,
1李遇梅,
1顾文涛,
1许辉
1 江苏大学附属医院皮肤科，江苏 镇江 212001 |
| 通讯: |
许辉
Email: xuhuiraian@sina.com.cn |
| DOI: | 10.3978/j.issn.2095-6959.2021.05.022 |
| 基金: | 江苏省妇幼健康科研项目(F201717)；镇江市社会发展项目(SH2018032)；江苏省预防医学课题(Y2018107)。 |
摘要
人乳头瘤病毒(human papillomavirus，HPV)是乳多空病毒科的一种具有圆形二十面体结构的双链DNA病毒。近年来，基于第二代测序技术的病毒宏基因组学逐渐兴起，它可以直接获得特定环境中的全部病毒核酸信息，并对其进行序列进化分析。可用于研究环境中病毒的多样性、未知病毒的鉴定、实时监测病毒的变异情况以及发现可能存在的新病毒。本文主要就近年来病毒宏基因组学及其相关检测方法在HPV感染相关性疾病的研究进行综述，以期为HPV相关疾病的检测提供新的思路。
关键词：
病毒宏基因组学；第二代测序技术；人乳头瘤病毒；生殖器疣；头颈部肿瘤
Research advances of viral metagenomics in HPV infection related diseases
CorrespondingAuthor: XU Hui Email: xuhuiraian@sina.com.cn
DOI: 10.3978/j.issn.2095-6959.2021.05.022
Foundation: This work was supported by Maternal and Child Health Project of Jiangsu Province (F201717), Zhenjiang Social Project (No. SH2018032), and the Phylaxiology Project of Jiangsu Province (No. Y2018107), China.
Abstract
Human papillomavirus (HPV) is a circular icosahedral double-stranded DNA virus, belonging to Papovaviridae. In recent years, viral metagenomics based on the second generation sequencing technology is gradually emerging, which can obtain all the viral nucleic acid information directly in a specific environment and carry out sequential evolution analysis. It can be used to study the diversity of virus in the environment, identify unknown virus, monitor the variation of virus in real time and find new virus that may exist. In this paper, we review the recent studies of viral metagenomics and related detection methods in HPV infection related diseases, in order to provide new ideas for the detection of HPV related diseases.
Keywords：
viral metagenomics; the second generation sequencing technology; human papillomavirus; genital warts; head and neck neoplasms