Objective: To explore the effect of BC-1215 on TNBS-induced colitis in mice. Methods: Balb/c mice were randomly divided into four groups. Two groups were given TNBS enema to induce colitis, and then were intraperitoneally injected with BC-1215 and PBS, respectively. The other two groups were given an equal volume of 50% ethanol enema, and were also injected with BC-1215 or PBS intraperitoneally as a negative control group. Colon tissues were collected 5 days later, HE staining was used to detect pathological damage, and qRT-PCR was used to detect the expression of pro-inflammatory cytokines (IL-6, TNF-α, IL-1β and IL-23), Th1 cells expressed IFN-γ and T-bet, and Th17 cells expressed IL-17A and RORgγt. Results: BC-1215 can significantly alleviate the severity of TNBS-induced colitis, characterized by less body weight loss, moderate colon shortening, slight histological damage and low lower pro-inflammatory cytokines expression in mice. Conclusion: BC-1215 has protective effects on TNBS-induced colitis in mice and has potential therapeutic effects on colitis.