1 江苏大学附属人民医院消化科，江苏 镇江 212002
许亚平 Email: email@example.com
|基金:||江苏省重点研发计划 (社会发展) 项目 (BE2017692)。|
目的：探索F-box蛋白3(F-box protein 3，FBXO3)抑制剂BC-1215对三硝基苯磺酸(trinitrobenzene sulfonic acid，TNBS)诱导的小鼠肠黏膜炎症反应的作用。方法：将Balb/c小鼠随机分为4组，每组10只，2组给予TNBS灌肠诱导结肠炎(实验组)，其中1组给予BC-1215腹腔注射，另1组给予磷酸缓冲盐溶液(PBS)腹腔注射；另外2组给予等体积50%乙醇灌肠作为阴性对照组，亦分别给予BC-1215或PBS腹腔注射。5 d后收集结肠组织，通过对比体重、结肠缩短程度、结肠组织苏木精-伊红染色(hematoxylin-eosin staining，HE)及髓过氧化物酶活性评估BC-1215对肠黏膜炎症影响，使用荧光定量PCR(qRT-PCR)检测肠黏膜组织中炎症细胞因子IL-6，TNF-α，IL-1β，IL-23及Th1细胞和Th17细胞相关细胞因子(IFN-γ和IL-17A)和转录因子(T-bet和RORγt)表达。结果：BC-1215抑制TNBS诱导的小鼠体重下降、减轻小鼠结肠缩短程度和炎症细胞浸润，并且抑制小鼠结肠组织中炎症细胞因子表达。结论：BC-1215对TNBS诱导的小鼠结肠炎具有保护作用，对结肠炎具有潜在治疗作用。关键词： BC-1215；结肠炎；炎症细胞因子
BC-1215 inhibits trinitrobenzene sulfonic acid-induced colitis in mice
CorrespondingAuthor: YAO Jun Email: firstname.lastname@example.org
Foundation: This work was supported by Jiangsu Provincial Key Research and Development Program, China (BE2017692).
Objective: To explore the effect of BC-1215 on TNBS-induced colitis in mice. Methods: Balb/c mice were randomly divided into four groups. Two groups were given TNBS enema to induce colitis, and then were intraperitoneally injected with BC-1215 and PBS, respectively. The other two groups were given an equal volume of 50% ethanol enema, and were also injected with BC-1215 or PBS intraperitoneally as a negative control group. Colon tissues were collected 5 days later, HE staining was used to detect pathological damage, and qRT-PCR was used to detect the expression of pro-inflammatory cytokines (IL-6, TNF-α, IL-1β and IL-23), Th1 cells expressed IFN-γ and T-bet, and Th17 cells expressed IL-17A and RORgγt. Results: BC-1215 can significantly alleviate the severity of TNBS-induced colitis, characterized by less body weight loss, moderate colon shortening, slight histological damage and low lower pro-inflammatory cytokines expression in mice. Conclusion: BC-1215 has protective effects on TNBS-induced colitis in mice and has potential therapeutic effects on colitis.Keywords： BC-1215; colitis; inflammatory cytokines