MiRNA-92a靶向PTEN通过PI3K/AKT信号通路促进卵巢癌细胞转移
| 作者: |
1王秋宇,
1朱军义,
2邓巧子
1 南阳市中心医院妇产科，河南 南阳 473000 2 河南科技大学第一附属医院新区医院妇产科，河南 洛阳 471000 |
| 通讯: |
邓巧子
Email: dengqiaozi2114@aliyun.com |
| DOI: | 10.3978/j.issn.2095-6959.2020.07.005 |
| 基金: | 洛阳市应用技术研究与开发资金项目(1201052A2)。 |
摘要
目的：探讨miRNA-92a对卵巢癌细胞侵袭与迁移能力变化的影响和可能的作用机制。方法：RT-qPCR检测卵巢癌组织和卵巢癌细胞miRNA-92a的表达水平；转染miRNA-92a inhibitor/mimic后，Transwell小室法检测SKOV3与A2780细胞的侵袭与迁移能力；蛋白质印迹法检测SKOV3与A2780细胞中p-PI3k，p-Akt和PTEN蛋白水平的变化；预测miRNA-92a靶基因并经双荧光素酶报告基因验证。结果：MiRNA-92a在卵巢癌组织和卵巢癌细胞中高表达(P<0.05)；转染miRNA-92a inhibitor后，SKOV3与A2780细胞的侵袭和迁移能力明显受到抑制，同时p-PI3k和p-Akt蛋白水平均显著降低，PTEN蛋白水平显著升高(均P<0.05)；转染miRNA-92a mimic后，以上指标相反；miRNA-92a靶基因为PTEN，且过表达PTEN能逆转过表达miRNA-92a诱导的侵袭转移与p-PI3k，p-Akt表达(均P<0.05)。结论：MiRNA-92a直接结合靶基因PTEN，通过激活PI3k/Akt通路活性，促进卵巢癌细胞SKOV3的侵袭与迁移能力。
关键词：
卵巢癌；miRNA-92a；侵袭；迁移
MiRNA-92a promote metastasis in ovarian cancer cells through PI3K/Akt signaling pathway by targeting PTEN
CorrespondingAuthor: DENG Qiaozi Email: dengqiaozi2114@aliyun.com
DOI: 10.3978/j.issn.2095-6959.2020.07.005
Foundation: This work was supported by Luoyang Applied Technology Research and Development Project Foundation, China (1201052A2).
Abstract
Objective: To investigate the effects and the possible mechanisms of miRNA-92a on the abilities of cell invasion and migration in ovarian cancer cells. Methods: The expressions of miRNA-92a in the ovarian cancer tissues and ovarian cancer cells were detected by RT-qPCR. After transfection with miRNA-92a inhibitor or mimic, the abilities of SKOV3 and A2780 cell invasion and migration were determined by Transwell assay. The protein levels of p-PI3K, p-Akt and PTEN were measured by Western blotting. Further, miRNA-92a target gene was predicted and then be verified by dual-luciferase reporter system assay. Results: The expressions of miRNA-92a were significantly higher in the ovarian cancer tissue and ovarian cancer cells (P<0.05). After transfected with miRNA-92a inhibitor into SKOV3 and A2780 cells, the abilities of invasion and migration were dramatically reduced, the protein levels of p-PI3K and p-Akt were markedly decreased and PTEN was markedly increased (all P<0.05). However, after transfection with miRNA-92a mimic, the above indicators were contrary. Furthermore, PTEN was the direct target gene of miRNA-92a, and over-expression PTEN reversed the migration and invasion induced by over-expression miRNA-92a in SKOV3 and A2780 cells, and the high expression of p-PI3k and p-Akt (all P<0.05). Conclusion: MiRNA-92a promotes the abilities of invasion and migration in the SKOV3 and A2780 ovarian cancer cells though activating of PI3K/Akt pathway by direct targeting PTEN.
Keywords：
ovarian cancer; miRNA-92a; invasion; migration