1 汕头潮南民生医院妇产科，广东 汕头 515044
2 汕头大学医学院第一附属医院病理科，广东 汕头 515041
3 汕头潮南民生医院病理科，广东 汕头 515044
目的：探讨TGF-β1在胎膜早破(premature rupture of membranes，PROM)患者胎盘组织羊膜上皮细胞中的表达情况，进而阐述胎膜早破发生的可能分子机制，为PROM的诊断、治疗及预防提供新的思路。方法：97例胎膜早破患者分为早产胎膜早破(preterm premature rupture of membranes，PPROM)和足月胎膜早破(full-term premature rupture of membranes，TPROM)两组，其中PPROM 49例，TPROM 48例，匹配同一时间段内正常足月产患者31例为对照组。首先收集患者的临床及病理基本信息，进而利用胎盘组织行TGF-β1免疫组织化学染色，观察羊膜上皮细胞中TGF-β1的表达情况。结果：C反应蛋白在各组间存在差异，而年龄、白细胞计数、怀孕次数、分娩次数、分娩方式、组织学绒毛膜羊膜炎各组间差异无统计学意义(P>0.05)。免疫组织化学染色发现：在正常对照组、PPROM组及TPROM组均出现不同程度的TGF-β1表达，PPROM组及TPROM与对照组比较差异具有统计学意义(P=0.004)，而PPROM组与TPROM组比较差异无统计学意义(P=0.355)。结论：TGF-β1参与了PROM的发病过程，其作用的增强可能是发生PROM的病理基础。
Expression and significance of TGF-β1 in amnion epithelial cells of patients with premature rupture of membranes
This work was supported by the Shantou City Science and Technology Plan Project in 2018 [Shanfuke(2018) 78], Shantou City Science and Technology Plan Project in 2019 [Shanfuke(2019) 106], China .
Objective: To investigate the expression of TGF-β1 in amnion epithelial cells of placental tissue in patients with premature rupture of membranes (PROM), to explain the possible molecular mechanism of premature rupture of membranes, and to provide new ideas for the diagnosis, treatment and prevention of PROM. Methods: Ninety-seven patients with PROM were divided into a preterm premature rupture of membranes (PPROM) group (n=49) and a full-term premature rupture of membranes (TPROM) group (n=48). Thirty-one women with normal term delivery in the same period were selected as a control group. Firstly, the clinical and pathological basic information of the patients were collected, and then TGF-β1 immunohistochemical staining was performed on placental tissue to observe the expression of TGF-β1 in amniotic membrane epithelial cells. Results: There were differences in CRP among 3 groups, while there were no significant differences in age, white blood cell count, number of pregnancies, number of deliveries, mode of delivery, and histological chorioamnionitis. Immunohistochemical staining showed that there were different degrees of TGF-β1 expression in the 3 groups. Compared with the control group, the PPROM group and the TPROM group showed statistically significant differences (P=0.004), while the comparison between PPROM group and TPROM group showed no statistically significant differences (P=0.355). Conclusion: TGF-β1 plays a role in the pathogenesis of PROM, and its enhancement may be the pathological basis of PROM.
premature rupture of membranes; TGF-β1; immunochemistry; amniotic membrane