文章摘要

MiR-139-5p通过靶向NFAT抑制卵巢癌SKOV3细胞的生长、集落形成、侵袭和迁移

作者: 1朱 军义, 1郭 哲, 1王 双双, 2邓 巧子
1 南阳市中心医院妇科一病区,河南 南阳 473000
2 河南科技大学第一附属医院新区医院妇产科,河南 洛阳 471000
通讯: 邓 巧子 Email: 976275778@qq.com
DOI: 10.3978/j.issn.2095-6959.2020.04.001
基金: 洛阳市应用技术研究与开发资金项目(1201052A-2)。

摘要

目的:探究miR-139-5p对卵巢癌SKOV3细胞的生长、集落形成、侵袭能力以及迁移能力的影响及其机制。方法:采用qRT-PCR检测卵巢癌患者癌组织和卵巢癌细胞SKOV3中miR-139-5p表达情况。MiR-139-5p mimic转染SKOV3细胞,WST比色实验、集落形成实验和Transwell实验分别检测细胞的活性、集落形成、侵袭和迁移能力。采用TargetScan在线软件筛选miR-139-5p的潜在靶基因NFAT,并进一步验证。结果:MiR-139-5p在卵巢癌组织和SKOV3细胞中表达异常降低。MiR-139-5p过表达显著抑制SKOV3细胞的生长、集落形成、迁移及侵袭能力。NFAT是miR-139-5p的靶基因。过表达NFAT能逆转miR-139-5p过表达对SKOV3细胞集落形成、侵袭能力以及迁移能力与侵袭的抑制作用。结论:MiR-139-5p通过抑制靶基因NFAT来抑制卵巢癌SKOV3细胞的生长、集落形成、侵袭和迁移能力。
关键词: miR-139-5p;NFAT;卵巢癌;生长;转移

MiR-139-5p inhibits the growth, colony formation, invasion and migration of ovarian cancer SKOV3 cells by targeting NFAT

Authors: 1ZHU Junyi, 1GUO Zhe, 1WANG Shuangshuang, 2DENG Qiaozi
1 Departmen of Obstetrics and Gynecology, Nanyang City Center Hospital, Nanyang Henan 473000, China
2 Department of Obstetrics and Gynecology, First Affiliated Hospital of Henan University of Science and Technology, Luoyang Henan 471000, China

CorrespondingAuthor:DENG Qiaozi Email: 976275778@qq.com

Foundation: This work was supported by the Luoyang Applied Technology Research and Development Fund Project, China (1201052A-2).

Abstract

Objective: To investigate the effects of miR-139-5p on the growth, cell colony formation, invasion and migration ability of ovarian cancer SKOV3 cells and its mechanism. Methods: qRT-PCR was used to detect the expressions of miR-139-5p in ovarian cancer tissues and ovarian cancer cells SKOV3. miR-139-5p mimic was transfected into SKOV3 cells, cell viability, cell colony formation, invasion and migration were detected by WST colorimetric assay, colony formation assay and Transwell assay, respectively. TargetScan online software was used to screen the potential target NFAT of miR-139-5p, and further verify. Results: MiR-139-5p expression were abnormally decreased in human ovarian cancer tissues and SKOV3 cells. MiRNA-139-5p overexpression significantly reduced the growth, cell colony formation, migration and invasion of SKOV3 cells. NFAT was a target gene for miR-139-5p. Overexpression of NFAT could reverse the inhibition of miR-139-5p overexpression on SKOV3 cell colony formation, invasion ability, invasion and migration. Conclusion: MiR-139-5p inhibits the growth, cell colony formation, invasion and migration of ovarian cancer SKOV3 cells by targeting NFAT.
Keywords: miR-139-5p; NFAT; ovarian cancer; growth; metastasis