文章摘要

MiR-101在乳腺癌中的表达及其意义

作者: 1朱 蕾, 2杨 颖, 3卢 晶, 1缪 妍, 4陈 红, 5崔 少宁, 1翟 丽云, 4张 金库, 1王 春光
1 保定市第一中心医院麻醉科,河北 保定 071000
2 保定市第一中心医院乳腺外科,河北 保定 071000
3 保定市第一医院肿瘤外科,河北 保定 071000
4 保定市第一中心医院病理科,河北 保定 071000
5 保定市第一中心医院手术室,河北 保定 071000
通讯: 张 金库 Email: 843561234@qq.com
DOI: 10.3978/j.issn.2095-6959.2020.02.006
基金: 保定市科技计划项目(1941ZF041)。

摘要

目的:探讨miR-101在乳腺癌组织中的表达变化。方法:收集乳腺癌组织及对应的癌旁组织,以qRT-PCR方法测定miR-101在乳腺癌组织中的表达变化。在乳腺癌细胞中转染miR-101 mimics、mimics control,以qRT-PCR方法检测上调效果,MTT方法测定细胞增殖变化,流式细胞术测定细胞凋亡变化,Transwell小室测定细胞迁移和侵袭数目变化,蛋白质印迹法测定细胞中cleaved caspase-3和MMP-2蛋白表达变化。结果:miR-101在乳腺癌组织中的表达水平明显低于癌旁组织(P<0.05)。与miR-NC比较,miR-101细胞中miR-101表达水平升高,细胞增殖能力降低,细胞凋亡率升高,细胞侵袭和迁移数目下降,细胞内的cleaved caspase-3蛋白水平表达升高,MMP-2蛋白表达水平减少(P<0.05)。结论:MiR-101在乳腺癌组织中表达下调,上调miR-101抑制乳腺癌细胞增殖、侵袭和迁移并诱导细胞凋亡。
关键词: 乳腺癌;miR-101;侵袭;凋亡;增殖

Expression of miR-101 in breast cancer and its significance

Authors: 1ZHU Lei, 2YANG Ying, 3LU Jing, 1MIAO Yan, 4CHEN Hong, 5CUI Shaoning, 1ZHAI Liyun, 4ZHANG Jinku, 1WANG Chunguang
1 Department of Anesthesiology, First Central Hospital of Baoding, Baoding Hebei 071000, China
2 Department of Breast Surgery, First Central Hospital of Baoding, Baoding Hebei 071000, China
3 Department of Surgical Onclology, Baoding First Hospital, Baoding Hebei 071000, China
4 Baoding No. 1 Department of Pathology, First Central Hospital of Baoding, Baoding Hebei 071000, China
5 Operating Room, First Central Hospital of Baoding, Baoding Hebei 071000, China

CorrespondingAuthor:ZHANG Jinku Email: 843561234@qq.com

Foundation: This work was supported by the Baoding Science and Technology Plan Project, China (1941ZF041).

Abstract

Objective: To detect the expression of MiR 101 in breast cancer tissues. Methods: The expression of MiR 101 in breast cancer tissues and their adjacent tissues were detected by qRT-PCR. miR-101 mimics and mimics control were transfected into breast cancer cells. The up-regulation effect was detected by qRT-PCR, cell proliferation was measured by MTT, cell apoptosis was measured by flow cytometry, cell migration and invasion were measured by Transwell chamber, and the expression of cleaved caspase-3 and MMP-2 protein were measured by Western blot. Results: The expression level of miR-101 in breast cancer tissues was significantly lower than that in adjacent tissues (P<0.05). Compared with miR-NC, the expression of miR-101 decreased, cell proliferation ability decreased, cell apoptosis rate increased, cell invasion and migration decreased, the expression of cleaved caspase-3 protein increased, and the expression of MMP-2 protein decreased in miR-101 cells (P<0.05). Conclusion: The down-regulation of the expression of miR-101 in breast cancer tissue and up-regulation of miR-101 inhibit the proliferation, invasion and migration of breast cancer cells and induce apoptosis.
Keywords: breast cancer; miR-101; invasion; apoptosis; proliferation