文章摘要

基于TCGA数据库分析GADD45B基因在卵巢浆液性囊腺癌中的表达及其临床价值

作者: 1孙 鹤倩, 1贾 雪梅, 1徐 娟
1 南京医科大学附属妇产医院(南京市妇幼保健院)妇科,南京 210004
通讯: 徐 娟 Email: happy617@126.com
DOI: 10.3978/j.issn.2095-6959.2020.02.002
基金: 国家自然科学基金(81572556,81602285,81872126)。

摘要

目的:利用TCGA数据库探讨生长停滞和DNA损伤诱导基因β(growth arrest and DNA damage-inducible gene β,GADD45B)的基因表达与卵巢浆液性囊腺癌临床特征的相关性及其在卵巢浆液性囊腺癌预后分析中的价值。方法:下载TCGA数据库中604例卵巢浆液性囊腺癌患者的临床信息及307例卵巢浆液性囊腺癌患者的RNA测序数据,利用卡方检验和t检验分析GADD45B表达与卵巢浆液性囊腺癌临床病理特征的相关性,利用Kaplan-Meier法分析GADD45B表达与卵巢浆液性囊腺癌总生存时间的相关性,利用单因素和多因素COX回归分析GADD45B是否可以作为卵巢浆液性囊腺癌预后分析的独立分子标志物,利用qRT-PCR检测GADD45B在多药耐药卵巢癌细胞及对照卵巢癌细胞中的表达。结果:GADD45B表达与卵巢浆液性囊腺癌的国际妇产科联合会(International Federation of Gynecology and Obstetrics,FIGO)分期、肿瘤大小、肿瘤在卵巢的浸润情况、初次化学药物治疗效果及生存情况均显著相关,且与卵巢浆液性囊腺癌的总生存时间显著负相关;多因素COX回归分析示:只有年龄和初次化疗效果可以作为卵巢浆液性囊腺癌的独立分子标记,同时qRT-PCR发现GADD45B在多药耐药卵巢癌细胞中显著升高。结论:GADD45B在耐药卵巢癌细胞中显著升高,其高表达与卵巢浆液性囊腺癌预后不良及化疗耐药明显相关,可作为分析卵巢浆液性囊腺癌预后的有效分子标志物。
关键词: 卵巢浆液性囊腺癌;生长停滞和DNA损伤诱导基因β;TCGA;预后

Analysis for GADD45B gene expression in ovarian serous cystadenocarcinoma and its clinical value based on TCGA database

Authors: 1SUN Heqian, 1JIA Xuemei, 1XU Juan
1 Department of Gynecology, Women’s Hospital of Nanjing Medical University (Nanjing Maternity and Child Health Care Hospital), Nanjing 210004, China

CorrespondingAuthor:XU Juan Email: happy617@126.com

Foundation: This work was supported by the National Natural Science Foundation of China (81572556, 81602285,81872126).

Abstract

Objective: To analyze the relationship between growth arrest and DNA damage-inducible gene β (GADD45B) expression and the clinicopathological features as well as its prognostic value of ovarian serous cystadenocarcinoma patients by using the cancer genome atlas (TCGA) database. Methods: The clinical information of 604 cases and the mRNA sequencing data of 307 cases of ovarian serous cystadenocarcinoma patients were downloaded from TCGA. χ2 was used to analyze the association of GADD45B and the clinicopathological features of ovarian serous cystadenocarcinoma patients. Kaplan-Meier was used to analyze the relationship between the expression of GADD45B and the overall survival of ovarian serous cystadenocarcinoma patients. Univariate and multivariate COX regression was further used to analyze whether GADD45B was an independent prognosis marker of ovarian serous cystadenocarcinoma patients. Multivariate COX regression analysis showed that only age and primary chemotherapy could be used as independent markers for ovarian serous cystadenocarcinoma. qRT-PCR was used to detect the expression of GADD45B in the multidrug resistant ovarian cancer cell line and control ovarian cancer cell line. Results: GADD45B expression level was significantly associated with age, FIGO stage, tumor size, tumor invasion in ovary, primary chemotherapy effect and the survival of ovarian serous cystadenocarcinoma and negatively correlated with overall survival rate of ovarian serous cystadenocarcinoma. qRT-PCR indicated that GADD45B was significantly increased in the multidrug resistant ovarian cancer cell line. Conclusion: GADD45B was significantly increased in the multidrug resistant ovarian cancer cell line, and high expression of GADD45B is associated with poor prognosis of ovarian serous cystadenocarcinoma and can be used as an effective molecular marker for prognostic analysis of ovarian serous cystadenocarcinoma.
Keywords: ovarian serous cystadenocarcinoma; growth arrest and DNA damage-inducible gene β; TCGA; prognosis