Objective: To investigate the relationship between quantitative parameters of contrast-enhanced ultrasound (CEUS) and microvessel density (MVD), cervical lymph node metastasis in papillary thyroid carcinoma (PTC) tissues. Methods: Seventy-five PTC patients confirmed by surgical pathology in Affiliated Hospital of Henan Academy of Chinese Medicine during the period from September 2016 to January 2019 were enrolled. According to pathological results of cervical lymph nodes, they were divided into a metastasis group (24 cases) and a non-metastasis group (51 cases). CEUS was performed on them before operation. The related blood flow perfusion parameters of PTC nodules and surrounding normal thyroid tissues were obtained. PTC nodules and normal histopathological specimens were collected to obtain MVD by immunohistochemical staining. And statistical analysis was performed. Results: The time peak (TP) and mean transit time (MTT) of PTC nodules were significantly higher than those of surrounding normal tissues, while peak intensity (PI) and area under the curve (AUC) were significantly lower than those of surrounding normal tissues (P<0.05). And MVD was significantly lower than that of surrounding normal tissues (P<0.05). Spearman rank correlation analysis showed CEUS parameter of PTC nodules such as TP and MTT were not significantly correlated with MVD (P>0.05), while PI and AUC were significantly positively correlated with MVD (P<0.05). Compared with the non-metastasis group, PI and AUC were significantly increased in the metastasis group (P<0.05). There was no significant difference in TP or MTT (P>0.05), while MVD was significantly increased (P<0.05). The receiver operating characteristic (ROC) curve AUC of PI and AUC for predicting cervical lymph node metastasis were 0.864 and 0.877, respectively. After determining critical value, the sensitivities of PI and AUC for predicting cervical lymph node metastasis were all not less than 75.0%, and the specificities were all 88.2%. Multivariate logistic regression analysis showed that the number of lesions, tumor size, PI and AUC were independent risk factors for predicting cervical lymph node metastasis (P<0.05). Conclusion: The CEUS blood flow perfusion parameters PI and AUC are significantly correlated with MVD in PTC tissues, which are of certain predictive value for cervical lymph node metastasis. They can be used as a noninvasive method for preoperative PTC evaluation.