文章摘要

上皮细胞黏附分子与肝母细胞瘤患儿病理特征及预后的相关性

作者: 1姚 远
1 西安市儿童医院普外一科,西安 710003
通讯: 姚 远 Email: yaoyuan_xb@yeah.net
DOI: 10.3978/j.issn.2095-6959.2020.01.006

摘要

目的:研究上皮细胞黏附分子(epithelial cell adhesion molecule,Ep-CAM)表达与肝母细胞瘤患儿病理特征及预后的相关性。方法:以西安市儿童医院2015年1月至2017年1月收治的62例肝母细胞瘤患儿的肿瘤组织和癌旁组织为研究材料,采用免疫组织化学法检测Ep-CAM表达。分析Ep-CAM表达情况与患儿性别、年龄、病理分期、病理类型等病理特征的关系,并绘制患儿生存曲线,分析上皮细胞黏附分子表达水平与预后的相关性。结果:62例肝母细胞瘤肿瘤组织和癌旁组织Ep-CAM表达阳性率分别为75.81%和0.00%,差异有统计学意义(P<0.05)。肝母细胞瘤组织中Ep-CAM表达阳性与PRETEXT术前分期有关,与患儿年龄、性别、术前甲胎蛋白水平和病理类型无关。PRETEXT分期Ⅲ期和Ⅳ期患儿肿瘤组织中Ep-CAM阳性表达率显著高于Ⅰ期和Ⅱ期患儿(P<0.05)。Ep-CAM阳性和阴性患儿2年总生存率分别为34.04%(16/47)和86.67%(13/15),差异有统计学意义(P<0.05)。Log-rank分析Ep-CAM阳性和阴性患儿总的生存率差异有统计学意义(P<0.05)。结论:Ep-CAM蛋白在肝母细胞瘤组织中呈高表达,与PRETEXT分期存在相关性,是影响预后的关键因素,可能作为肝母细胞瘤诊断和预后评估的新靶点加以深入研究。
关键词: 肝母细胞瘤;上皮细胞黏附分子;病例特征;预后;免疫组织化学

Correlation between epithelial cell adhesion molecule and pathological features and prognosis of hepatoblastoma in children

Authors: 1YAO Yuan
1 Department of General Surgery I, Xi’an Children’s Hospital, Xi’an 710003, China

CorrespondingAuthor:YAO Yuan Email: yaoyuan_xb@yeah.net

Abstract

Objective: To study the correlation between the expression of epithelial cell adhesion molecule (Ep-CAM) and the pathological characteristics and prognosis of hepatoblastoma in children. Methods: Ep-CAM expression in 62 cases of hepatoblastoma from January 2015 to January 2017 was detected by immunohistochemistry. The relationships between the Ep-CAM expression and the pathological characteristics (such as gender, age, pathological stage, and pathological type) were analyzed. The survival curve was drawn to analyze the correlation between the expression level of Ep-CAM and prognosis. Results: The positive rates of Ep-CAM expression in 62 cases of hepatoblastoma were 75.81% and 0.00%, respectively (P<0.05). The positive expression of Ep-CAM in hepatoblastoma was related to PRETEXT stage, but not related to age, sex, preoperative alpha-fetoprotein level and pathological type of children. The positive expression rates of Ep-CAM in cancer tissues of children with PRETEXT stage III and IV were significantly higher than those in PRETEXT stage I and II (P<0.05). The 2-year overall survival rates of Ep-CAM positive and negative children were 34.04% (16/47) and 86.67% (13/15), respectively, with significant difference (P<0.05). Log-rank analysis showed that the overall survival rate of Ep-CAM positive and negative children was significantly different (P<0.05). Conclusion: Ep-CAM protein is highly expressed in hepatoblastoma tissues and correlates with PRETEXT staging. Ep-CAM protein is a key factor affecting the prognosis of hepatoblastoma. It may be a new target for diagnosis and prognosis evaluation of hepatoblastoma.
Keywords: hepatoblastoma; epithelial cell adhesion molecule; case characteristics; prognosis; immunohistochemistry