文章摘要

MiR-520a对胃癌SGC7901细胞增殖、侵袭、迁移以及顺铂药物敏感性的影响

作者: 1吴 越菲, 1徐 礼鹏, 2孙 燃, 1韦 永明
1 芜湖市第二人民医院介入科,安徽 芜湖 241000
2 芜湖市第二人民医院病理科,安徽 芜湖 241000
通讯: 韦 永明 Email: 1721385835@qq.com
DOI: 10.3978/j.issn.2095-6959.2020.01.001

摘要

目的:探讨miR-520a在胃癌中的表达以及对胃癌细胞生物学功能的影响。方法:通过real-time PCR检测miR-520a在胃癌组织和胃癌细胞中的表达。采用慢病毒在胃癌细胞SGC7901中过表达miR-520a,在体外观察miR-520a对胃癌细胞增殖、凋亡、侵袭和迁移以及对顺铂(DDP)敏感性的影响。并通过异种移植瘤实验观察过表达miR-520a对胃癌细胞在裸鼠体内生长的影响。结果:与癌旁正常组织相比,miR-520a在胃癌组织中的表达明显降低。与正常胃黏膜上皮细胞GES-1相比,miR-520a在胃癌SGC7901细胞中的表达明显降低,且在顺铂耐药SGC7901/DDP细胞中的表达更低。miR-520a过表达能够促进SGC7901细胞凋亡,抑制细胞增殖、侵袭和迁移。miR-520a过表达促进SGC7901细胞对DDP的药物敏感性增加。异种移植瘤实验发现miR-520a过表达的细胞在裸鼠体内形成的肿瘤生长速度减慢。结论:miR-520a可抑制胃癌发生与发展,提高胃癌细胞对顺铂的敏感性。
关键词: 胃癌;miR-520a;抑癌基因;顺铂敏感性

Effect of miR-520a on proliferation, invasion, migration and cisplatin sensitivity in gastric cancer SGC7901 cells

Authors: 1WU Yuefei, 1XU Lipeng, 2SUN Ran, 1WEI Yongming
1 Department of Invasive Technology, Second People’s Hospital of Wuhu, Wuhu Anhui 241000, China
2 Department of Pathology, Second People’s Hospital of Wuhu, Wuhu Anhui 241000, China

CorrespondingAuthor:WEI Yongming Email: 1721385835@qq.com

Abstract

Objective: To investigate the expression of miR-520a in gastric cancer and its effect on the biological function in gastric cancer cells. Methods: The expression of miR-520a in gastric tissues and gastric cells was detected by real-time PCR. Lentivirus was used to over-express miR-520a in gastric cancer SGC7901 cells, and the effect of miR-520a on proliferation, apoptosis, invasion and migration of gastric cancer cells and the sensitivity to cisplatin (DDP) was evaluated in vitro. In addition, the effect of miR-520a over-expression on the growth of xenograft tumor was observed in vivo. Results: The expression of miR-520a in gastric cancer tissues was significantly lower than that in the adjacent normal tissues. Compared with normal gastric mucosal epithelial GES-1 cells, the expression of miR-520a was significantly decreased in gastric cancer SGC7901 cells and even lower in cisplatin resistant SGC7901/DDP cells. MiR-520a over-expression promoted apoptosis and inhibited cell proliferation, invasion and migration in SGC7901 cells. MiR-520a over-expression promoted the drug sensitivity of SGC7901 cells to DDP. Xenograft tumor experiment data showed that the miR-520a over-expression slowed down the tumor growth in nude mice. Conclusion: MiR-520a can inhibit the occurrence and development of gastric cancer and enhance the sensitivity of gastric cancer cells to cisplatin.
Keywords: gastric cancer; miR-520a; tumor suppressor; cisplatin sensitivity