文章摘要

免疫胶体金电镜技术在医学研究中的应用

作者: 1石 佼玉, 1张 志刚
1 复旦大学上海医学院基础医学院病理学系,上海 200032
通讯: 张 志刚 Email: zzg@shmu.edu.cn
DOI: 10.3978/j.issn.2095-6959.2019.09.038

摘要

免疫电镜技术是免疫化学技术与电镜技术结合的产物,是通过标记的抗体与特定的抗原结合来观察和研究超微结构水平组织细胞形态和功能。根据标记方法的不同,分为免疫铁蛋白技术、免疫酶标技术和免疫胶体金技术,其中应用较多的是胶体金技术。在电镜水平,金颗粒具有很高的电子密度,清晰可辨,因此利用胶体金标记的二抗,与相应一抗结合,从而将目标抗原标记。免疫电镜胶体金标记法近年来被成功地应用于医学生物学的各个方面,并取得了长足的进展,为解决一些医学上的微观结构问题提供了很好的帮助。
关键词: 免疫电镜;胶体金;肾病;肿瘤

Application of immune colloidal gold electron microscope technology in medical research

Authors: 1SHI Jiaoyu, 1ZHANG Zhigang
1 Department of Pathology, School of Basic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China

CorrespondingAuthor:ZHANG Zhigang Email: zzg@shmu.edu.cn

Abstract

Immunoelectron microscope technology is a method with combination of immunochemical technology and electron microscope, which can be used to observe and study the morphology and function of the cells and organs at the ultrastructural level by binding the labeled antibodies to specific antigens. It is divided into three types including immuno-iron protein technology, immuno-enzyme labeling technology and immune colloidal gold technique depending on different labeling methods. Among them, immune colloidal gold technique is more common to be used. At the electron microscope level, the gold particle has a high electron density and is clear distinguishable. Therefore, the secondary antibody combined with gold particles is used to react with the primary antibody to label the targeted antigen. In last decade, immune colloidal gold technique has made great progress for successfully applying to various aspects of cell biology, which provides much help for solving some medical problems under ultrastructural level.
Keywords: immunoelectron microscope; colloidal gold; kidney disease; tumors