1 广州医科大学附属肿瘤医院肿瘤研究所，广州恶性肿瘤治疗转化医学重点实验室，广州 510095
目的：探讨miR-155在转化生长因子-β1(transforming growth factor-β1，TGF-β1)诱导的人前列腺癌细胞PC3中的生物学作用。方法：TGF-β1处理PC3细胞，相差倒置显微镜下观察细胞形态学变化；通过Transwell试验检测细胞的迁移能力；实时荧光定量PCR(quantitative real-time polymerase chain reaction，qRT-PCR)和蛋白质印迹法检测TGF-β1诱导的PC3细胞纤维连接蛋白(fibronectin，FN)的表达情况；qRT-PCR检测miR-155的表达水平；干扰miR-155检测TGF-β1诱导的PC3细胞FN的表达及细胞迁移能力的变化。结果：TGF-β1诱导PC3细胞发生形态改变并增强其迁移能力，且FN的表达显著上调；TGF-β1诱导的miR-155表达呈现时间依赖性；干扰miR-155使TGF-β1诱导的FN表达减少，细胞形态及迁移能力在一定程度上发生逆转。结论：miR-155在TGF-β1诱导的前列腺癌细胞PC3中高表达，可能间接上调FN的表达从而促进细胞迁移，可为前列腺癌的治疗提供理论依据。
Role of miRNA-155 in TGF-β1-induced human prostate cancer cells
This work was supported by the Natural Science Foundation of Guangdong Province (2017A030313500), Guangzhou Science and Technology General Project (201707010381)
Objective: To investigate the biological roles of miR-155 in transforming growth factor-β1 (TGF-β1)-treated human prostate cancer cells PC3. Methods: PC3 cells were treated with TGF-β1. The morphological changes were observed under phase-contrast microscopy. Migration of PC3 cells was detected by Transwell assay. The expression of FN was assessed by quantitative real-time polymerase chain reaction (qRT-PCR), Western blotting and immunofluorescence staining. miR-155 treated by TGF-β1 were analyzed by qRT-PCR. After interfering with miR-155 in TGF-β1-treated PC3 cells, qRT-PCR and Western blotting were used to detect FN expression. Migration of TGF-β1-treated PC3 cells interfered with miR-155 was detected by Transwell assay. Results: TGF-β1 treatment could induce morphological alteration of PC3 cells from compact, cobblestone morphology into spindle-shaped, ﬁbroblast-like morphology. Transwell assay showed that TGF-β1 significantly increased the migration of PC3 cells. qRT-PCR, Western blotting and immunofluorescence staining demonstrated that TGF-β1 up-regulated the expression of FN. The expression of miR-155 induced by TGF-β1 was in a time-dependent manner. Introduction of miR-155 inhibitor partly attenuated the expression of FN in mRNA and protein levels, and inhibited the change of cell morphology and migration in TGF-β1 induced PC3 cells. Conclusion: miR-155 is up-regulated by TGF-β1 in PC3 cells, which may affect cell migration by increasing FN expression. It may have implications for treatment of human prostate cancer.
miRNA-155; transforming growth factor-β1; human prostate cancer; fibronectin