1 武汉大学人民医院肿瘤中心，武汉 430060
2 武汉大学人民医院病理科，武汉 430060
目的：基于生物信息数据挖掘阐明G蛋白偶联受体C家族5A基因(G protein-coupled receptor family C，member 5，grou p A，GPRC5A)在胰腺癌中的表达及临床意义。方法：检索Oncomine， TCGA，Human Protein Atlas等基因数据库，分析GPRC5A在胰腺癌中的表达差异，并分析其在不同肿瘤中的表达。采用Western印迹技术在武汉大学人民医院小样本队列中验证GPRC5A在胰腺癌及癌旁组织中的蛋白表达水平，采用Kaplan-Meier进行患者生存分析，并对GPRC5A与靶向药物敏感性关系进行分析。结果：对Oncomine数据库中有差异表达的295项研究数据进行差异性分析，发现胰腺癌组织GPRC5A基因的表达明显高于正常胰腺组织。对GPRC5A在不同肿瘤组织中表达的4 184项研究进行荟萃分析，发现GPRC5A在胰腺癌组织中显著表达增高。通过生存分析发现高表达GPRC5A胰腺癌患者生存期明显低于低表达者，高表达患者预后更差。此外，GPRC5A表达与靶向药物厄洛替尼的药物敏感性有一定关联。结论：GPRC5A在胰腺癌组织中呈现高表达，与患者预后显著相关，其有可能成为胰腺癌诊断及药物治疗的新靶点。关键词： 胰腺癌；GPRC5A；Oncomine数据库；TCGA数据库；Human Protein Atlas数据库
Expression and significance of GPRC5A in pancreatic cancer utilizing datasets
CorrespondingAuthor:TAO Weiping Email: firstname.lastname@example.org
Foundation: This work was supported by the National Natural Science Foundation of China (81302133).
Objective: To explore the expression of G protein-coupled receptor family C, member 5, group A (GPRC5A) and its prognostic role in pancreatic cancer based on datasets. Methods: The data of the GPRC5A in pancreatic cancer were extracted from the online open Oncomine, TCGA and Human Protein Atlas databases. Then we explored the difference in various cancers and verified the expression value of GPRC5A by immunohistochemical study with clinical pancreatic cancer tissues. The prognostic value of GPRC5A in pancreatic cancer was analyzed by Kaplan-Meier Plotter. In addition, the association between sensitive of molecule-targeting agents and GPRC5A in pancreatic cancer was researched. Results: A total 295 studies were obtained from Oncomine, and the results were found that the expression of GPCR5A in pancreatic cancer was significantly higher than normal pancreatic tissues, which were also verified by our immunohistochemical experiment. Compared with other types of tumor in 4 184 samples, the expression of GPCR5A was significantly higher in pancreatic cancer. Survival analysis of pancreatic cancer showed that the patients with higher level of GPCR5A showed a worse long-term prognosis. Furthermore, the correlation between the clinical sensitive of Erlotinib and elevated GPCR5A were found, which suggested that the GPCR5A may affect the regulation of chemosensitivity of Erlotinib. Conclusion: GPCR5A is found highly expressed in pancreatic cancer tissue and its expression has a significant impact on the prognosis and Erlotinib related chemotherapy, which is expected to be a potential molecular diagnostic and therapeutic target.Keywords： pancreatic cancer; GPRC5A; Oncomine; TCGA; Human Protein Atlas