文章摘要

七氟醚对乳腺癌细胞侵袭、迁移能力的影响及作用机制

作者: 1张 玉河, 1王 立力, 1王 志涛, 1许 仙华, 1赵 坤
1 延安大学咸阳医院麻醉科,陕西 咸阳 712000
通讯: 王 立力 Email: 23707080@qq.com
DOI: 10.3978/j.issn.2095-6959.2019.02.001

摘要

目的:探究七氟醚与乳腺癌MDA-MB-231细胞侵袭、迁移的关系以及作用机制。方法:采用不同浓度七氟醚处理MDA-MB-231细胞6 h,分为对照组、1.7%给药组、3.4%给药组、5.1%给药组,划痕实验和Transwell小室法观察细胞的迁移、侵袭能力,采用Western印迹法检测基质金属蛋白酶-2(matrix metalloprotease-2,MMP-2)、MMP-9、钙黏蛋白E(E-cadherin)、β-连环蛋白(β-catenin)的水平。结果:与对照组相比,七氟醚处理MDA-MB-231细胞迁移率和侵袭细胞数随着浓度的增加而降低(P<0.05)。MDA-MB-231细胞经七氟醚干预后,细胞中MMP-2,MMP-9,E-cadherin, β-catenin蛋白水平显著降低(P<0.05),且随着浓度的增加逐渐降低;Wnt/β-catenin信号通路激活剂LiCl作用于5.1%七氟醚干预6 h的细胞,细胞中β-catenin和E-cadherin蛋白水平明显增加,侵袭、迁移能力显著提高。结论:七氟醚可能是通过调控Wnt/β-catenin信号通路影响MMP-2,MMP-9蛋白水平,抑制乳腺癌MDA-MB-231细胞的侵袭、迁移能力。该效应具有浓度依赖性,为乳腺癌的治疗提供新的理论基础。
关键词: 乳腺癌;七氟醚;MDA-MB-231细胞;Wnt/β-catenin信号通路

Effect of sevoflurane on the metastasis ability of breast cancer cells and its mechanism

Authors: 1ZHANG Yuhe, 1WANG Lili, 1WANG Zhitao, 1XU Xianhua, 1ZHAO Kun
1 Department of Anesthesiology, Xianyang Hospital of Yanan University, Xianyang Shaanxi 712000, China

CorrespondingAuthor:WANG Lili Email: 23707080@qq.com

Abstract

Objective: To investigate the relationship between sevoflurane and invasion, migration of breast cancer MDA-MB-231 cells and its mechanism. Methods: MDA-MB-231 cells were treated with different concentrations of sevoflurane for 6 h. The experiment was divided into a control group, a 1.7% administration group, a 3.4% administration group, and a 5.1% administration group. The migration and invasion ability of cells was observed by scratch test and Transwell chamber assay. The protein levels of matrix metalloprotease-2 (MMP-2), MMP-9, E-cadherin and β-catenin were examined by Western blot. Results: Compared with the control group, the migration and invasion of MDA-MB-231 cells treated with sevoflurane significantly decreased (P<0.05). The levels of MMP-2, MMP-9, E-cadherin, and β-catenin in MDA-MB-231 cells treated with sevoflurane were significantly decreased with an increase of concentration (P<0.05). The LiCl, a Wnt/β-catenin signaling pathway activator, which interacted with 5.1% sevoflurane for 6 h, and the levels of β-catenin and E-cadherin were significantly increased, and the invasion and migration ability were significantly increased. Conclusion: Sevoflurane can inhibit the invasion and migration of breast cancer MDA-MB-231 cells in a concentration-dependent by regulating Wnt/β-catenin signaling pathway and MMP-2 and MMP-9 protein levels, providing a new theoretical basis for the treatment of breast cancer.
Keywords: breast cancer; sevoflurane; MDA-MB-231 cells; Wnt/β-catenin signaling pathway