MicroRNA-197调控上皮-间充质转化对乳腺癌侵袭和迁移的影响
| 作者: |
1沈庆林,
1宋启斌,
1章必成,
1姚颐,
1彭敏
1 武汉大学人民医院肿瘤中心，武汉 430060 |
| 通讯: |
宋启斌
Email: qibinsong@163.com |
| DOI: | 10.3978/j.issn.2095-6959. 2018.12.001 |
| 基金: | 国家自然科学基金(81472748)。 |
摘要
目的：探讨微小RNA-197(miR-197)抑制乳腺癌细胞迁移和侵袭的能力，以及阻断上皮-间充质转化(epithelial-mesenchymal transition，EMT)过程的机制。方法：构建miR-197过表达载体(miR-197 mimics)，分别转染MDA-MB-231和MCF-7细胞，并设对照组；Real-time PCR分别检测以上各组细胞miR-197的表达水平变化；利用Transwell实验和划痕实验对乳腺癌细胞侵袭和迁移能力进行检测；Western印迹法检测过表达miR-197后对EMT相关标志物E-cadherin，snail和vimentin表达的影响。结果：MiR-197可以抑制乳腺癌细胞MDA-MB-231和MCF-7的迁移和侵袭能力；转染miR-197 mimics后，E-cadherin表达降低，snail和vimentin表达增加。结论：MiR-197有可能作为乳腺癌临床治疗的新靶点。
关键词：
乳腺癌；微小RNA-197；上皮-间充质转化；侵袭；迁移
Effects of microRNA-197 regulating epithelial mesenchymal transition on invasion and migration in breast cancer
CorrespondingAuthor: SONG Qibin Email: qibinsong@163.com
DOI: 10.3978/j.issn.2095-6959. 2018.12.001
Foundation: This study was supported by the National Natural Science Foundation of China (81472748).
Abstract
Objective: To investigate the ability of microRNA-197 (miR-197) to inhibit the migration and invasion of breast cancer cells and the mechanism of blocking the process of epithelial-mesenchymal transition (EMT). Methods: The miR-197 mimics vector was constructed, and transfected into MDA-MB-231 and MCF-7 cell, the control group were transfected with empty vectors. The expression of miR-197 was detected by real-time PCR. The transwell system and the wound healing were used to assess the invasion and migration ability of breast cancer cell line MDA-MB-231 and MCF-7. Western blot was used to assay the expression of E-cadherin, Snail and Vimentin, which were the markers of EMT. Results: MiR-197 inhibited the migration and invasion of breast cancer cells MDA-MB-231 and MCF-7. Transfection of miR-197 mimics reduced the expression of E-cadherin and increased the protein expression of snail and vimentin. Conclusion: MiR-197 may be a new target for clinical treatment of breast cancer.
Keywords：
breast cancer; microRNA-197; epithelial-mesenchymal transition; invasion; migration