Objective: To investigate the effect of anti-resistance exercise combined with transcranial direct current stimulation on limb movement function of stroke patients with hemiplegia. Methods: A total of 98 cases were randomly divided into three groups in stroke patients in the stable period from January 2016 to April 2017 in our hospital rehabilitation, the conventional group of 33 patients with conventional rehabilitation management scheme, the electrical stimulation group 32 cases with “conventional rehabilitation + transcranial direct current stimulation” scheme, combined group of 33 cases with routine rehabilitation combined with “transcranial direct current stimulation + resistance exercise program”. The changes of limb motor function, activities of daily living, neurological impairment, serum neuropeptide Y (NPY) and IL-10 in three groups were compared. Results: After intervention, the Fugl-Meyer Motor Scale (FMMS) scores and activity of daily living (ADL) scores of the combined group were significantly higher than those of the control group and the electric stimulation group. The Chinese Stroke Scale (CSS) scores of the combined group were significantly lower than those of the other two groups, while the NPY and IL-10 levels of the combined group were significantly lower than those of the other two groups. Conclusion: Resistance exercise combined with transcranial direct current stimulation can effectively improve limb movement function and improve neurological deficit symptoms in patients with hemiplegia after stroke, and all of them are noninvasive operation, which is worthy of further promotion in clinical practice.