个体化慢性结肠电刺激对慢传输型便秘模型犬排便及胃肠传输的影响
| 作者: |
1,2朱丹,
1李艳梅,
1,2姚树坤,
1陈朔,
3陈少轩,
4杜玉惠
1 中日友好医院消化内科，北京 100029 2 中国医学科学院北京协和医学院研究生院，北京 100730； 3 中日友好医院普外科，北京 100029 4 常州瑞神安医疗器械有限公司，江苏 常州 213000 |
| 通讯: |
姚树坤
Email: yaosk123@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.04.006 |
| 基金: | 国家自然科学基金(81541014)。 |
摘要
目的：探讨个体化慢性结肠电刺激对慢传输型便秘(slow transit constipation，STC)模型犬排便情况和胃肠传输功能的影响。方法：纳入8只比格犬为研究对象，随机分为电刺激组和假刺激组，所有犬行开腹手术植入结肠电刺激系统，待恢复后进入干预期。采用低纤维素饮食联合复方地芬诺酯和盐酸阿洛司琼进行STC造模，共计5周，第2周同时进行个体化结肠电刺激/假刺激(只模拟动作，不开启电刺激)，观察和记录犬排便次数、大便性状，干预完成后测定胃肠传输时间(gastrointestinal transit time，GITT)，比较2组间差别。结果：8只比格犬均顺利完成所有实验项目，所筛选的刺激参数间存在个体差异。与假刺激组相比，电刺激可以增加排便频率[(3.08±0.31)次/d vs (2.43±0.43)次/d，P=0.049]、缓解排便性状(3.30±0.32 vs 2.55±0.47，P=0.037)、缩短GITT[(32.98±2.38) h vs (40.85±1.31) h，P=0.001]。结论：个体化慢性结肠电刺激能够缓解STC模型犬的便秘症状、促进胃肠蠕动。
关键词：
慢传输型便秘；动物模型；结肠电刺激；胃肠传输
Effects of individualized chronic colonic electrical stimulation on defecation and gastrointestinal transit in slow transit constipation dogs
CorrespondingAuthor: YAO Shukun Email: yaosk123@126.com
DOI: 10.3978/j.issn.2095-6959.2018.04.006
Foundation: This work was supported by the National Natural Science Foundation of China (81541014).
Abstract
Objective: To explore the effect of individualized chronic colonic electrical stimulation on defecation and gastrointestinal transit in slow transit constipation dogs. Methods: Eight beagles were included in this study, which were randomly divided into an electrical stimulation group and a sham stimulation group. All dogs underwent operation to implant the colonic electrical stimulation system, and given intervention when recovered. A combination of low fiber diet, compound diphenoxylate and aldosterone hydrochloride in oral administration for consecutive 5 weeks, individual colonic electrical stimulation/sham stimulation (simulated actions only, no electrical stimulation) were given from the second week, defecation and gastrointestinal transit time (GITT) were assessed. Results: Eight dogs went through all experiment items. Individual differences were found among the selected individualized parameters. Compared with the sham stimulation group, electrical stimulation could increase the frequency of defecation [(3.08±0.31) times/d vs (2.43±0.43) times/d, P=0.049], relieve the stool consistency (3.30±0.32 vs 2.55±0.47, P=0.037), shorten GITT [(32.98±2.38) h vs (40.85±1.31) h, P=0.001]. Conclusion: The individualized chronic colonic electrical stimulation can relieve the constipation symptoms and promote the gastrointestinal peristalsis in STC model dogs.
Keywords：
slow transit constipation; animal model; colonic electrical stimulation; gastrointestinal transit