结肠癌中miR-1231表达变化的临床意义
| 作者: |
1顾园,
2龚攀
1 鄂东医疗集团黄石市中心医院，湖北理工学院附属医院消化内科，湖北 黄石 435000 2 鄂东医疗集团黄石市中心医院，湖北理工学院附属医院肿瘤外科，湖北 黄石 435000 |
| 通讯: |
龚攀
Email: gongpan82@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.01.003 |
| 基金: | 湖北省卫生和计划生育委员会2015–2016年度科研立项基金一般项目(2015MB180)。 |
摘要
目的：观察结肠癌患者组织标本中miR-1231表达的改变，并探讨其相关的临床意义。方法：取鄂东医疗集团黄石市中心医院2016年2月至2017年8月收治的结肠癌患者94例，取癌旁非肿瘤组织作为对照。采用qRT-PCR检测miR-1231的表达。分析miR-1231表达的变化与各临床指标间的相关性。结果：结肠癌组织中miR-1231的2−△Ct值为0.146±0.018，显著低于对照组织的0.282±0.047(P<0.01)。miR-1231的2−△Ct值结肠癌患者的性别、年龄、肿瘤部位、肿瘤大小无相关性(P>0.05)。miR-1231的2−△Ct值在TNM临床III，IV期患者为0.125±0.014，显著低于I，II期患者的0.161±0.028(P<0.05)。miR-1231的2−△Ct值在中、低分化患者为0.131±0.015，显著低于高分化的0.174±0.032(P<0.05)。miR-1231值在淋巴结有转移患者为0.125±0.014，显著低于无转移的0.154±0.020(P<0.05)。Pearson相关分析显示miR-1231表达与患者的临床TNM分析(r=−0.361，P<0.05)、肿瘤分化(r=−0.342，P<0.05)、淋巴结是否转移(r=−0.354，P<0.05)呈负相关。结论：结肠组织中miR-1231低表达，其表达变化可能参与结肠癌的发生、发展、侵袭和转移过程。
关键词：
结肠癌；miR-1231；qRT-PCR
Clinical significance of changing in miR-1231 expression in colon cancer
CorrespondingAuthor: GONG Pan Email: gongpan82@163.com
DOI: 10.3978/j.issn.2095-6959.2018.01.003
Foundation: This work was supported by the Project of Hubei Provincial 2015–2016 Health and Family Planning Commission of Science and Research Fund, China (2015MB180).
Abstract
Objective: To detect miR-1231 expression in colon cancer and explore its clinical significance. Methods: Ninety-four patients of colon cancer were enrolled in this study. The non-cancerous tissues beside the tumor were taken as controls. QRT-PCR analysis was used to detect miR-1231 expression. Results: MiR-1231 expression in the colon cancer tissue was 0.146±0.018, which was lower than that of 0.282±0.047 in the control tissue (P<0.01). There was no significant correlation of miR-1231 with colon cancer patients’ gender, age, tumor location, and tumor size (P>0.05). MiR-1231 expression in III and IV stage group was 0.125±0.014, which was lower than that of 0.161±0.028 in I and II stage group (P<0.05). MiR-1231 expression in median and low differentiation group was 0.131±0.015, which was lower than that of 0.174±0.032 in well differentiation group (P<0.05). MiR-1231 expression in lymph node metastasis group was 0.125±0.014, which was lower than that of 0.154±0.020 in non-lymph node metastasis group (P<0.05). Pearson correlation analysis showed that the expression of miR-1231 was negatively correlated with clinical TNM analysis (r=−0.361, P<0.05), tumor differentiation (r=−0.342, P<0.05), lymph node metastasis (r=−0.354, P<0.05). Conclusion: Low expression of miR-1231 is found in colon cancer, which may be related to clinical stages, lymph node metastasis, and tumor differentiation of bladder cancer.
Keywords：
colon cancer; miR-1231; qRT-PCR