文章摘要

miRNA-27b在骨肉瘤中的表达及其临床意义

作者: 1张 其川, 2王 素芳, 1王 云峰, 1杜 云飞, 1付 新生
1 新乡市中心医院骨2科,河南 新乡 453000
2 新乡市中心医院消毒供应中心,河南 新乡 453000
通讯: 张 其川 Email: master-zqc@163.com
DOI: 10.3978/j.issn.2095-6959.2017.12.020

摘要

目的:探讨微小RNA(miRNA)-27b在骨肉瘤中的表达情况及诊断和预后价值。方法:选取经病理证实为骨肉瘤的患者血样51例,年龄和性别相匹配的51例健康者血样作为对照组。采用实时定量聚合酶链反应(qRT-PCR)检测骨肉瘤患者及健康对照组血清miR-27b表达水平。结果:相较于健康对照组,骨肉瘤患者血清中miR-27b表达水平明显升高(P<0.001)。miR-27b的高水平表达与较大的肿瘤体积(P=0.008),较高的TNM分级(P=0.001)及远处转移(P=0.001)相关。受试者工作曲线(ROC)提示血清miR-27b水平对骨肉瘤患者具有诊断价值。曲线下面积(AUC)为0.863,其中敏感度为77.8%,特异度为86.7%。Kaplan-Meier曲线分析表明miR-27b高水平表达组5年生存率较低。此外,单变量和多变量Cox回归分析表明miR-27b是骨肉瘤患者预后的一个独立危险因素(P<0.001和P=0.006)。结论:血清miR-27b水平可能成为骨肉瘤患者诊断及判断预后的生物标志物。
关键词: miR-27b;骨肉瘤;诊断;预后;生物标志物

Expression and clinical significance of circulating microRNA-27b in patients with osteosarcoma

Authors: 1ZHANG Qichuan, 2WANG Sufang, 1WANG Yunfeng, 1DU Yunfei, 1FU Xinsheng
1 Second Department of Orthopaedics, Xinxiang Central Hospital, Xinxiang Henan 453000, China
2 Central Sterile Supply Department, Xinxiang Central Hospital, Xinxiang Henan 453000, China

CorrespondingAuthor:ZHANG Qichuan Email: master-zqc@163.com

Abstract

Objective: To investigate the application of serum miR-27b in diagnosis and prognosis of patients with osteosarcoma. Methods: Using quantitative reverse transcription polymerase chain reaction (qRT-PCR) technique, we assessed expression of studied miR-27b in serum samples of 51 pairs of osteosarcoma patients and corresponding healthy individuals. Results: Serum levels of miR-27b were found to be significantly higher in osteosarcoma patients than those in healthy controls (P<0.001). Moreover, elevated serum levels of miR-27b in osteosarcoma patients were correlated with the large tumor size (P=0.008), advanced TNM stages (P=0.001) and distant metastasis (P=0.001). Receiver operating characteristic (ROC) curve analyses suggested that serum miR-27b had significant diagnostic value for osteosarcoma patients. It yielded an area under the curve (AUC) of ROC of 0.863 with 77.8% sensitivity and 86.7% specificity in distinguishing osteosarcoma patients from control subjects. Furthermore, Kaplan-Meier curve analysis indicated that osteosarcoma patients with a high serum miR-27b level had a significantly shortened overall survival (P<0.001). In addition, univariate and multivariable Cox regression analysis revealed that serum miR-27b level was an independent risk factor for overall survival (P<0.001 and P=0.006, respectively). Conclusion: Therefore, serum levels of miR-27b may serve as a potential non-invasive biomarker for diagnosis and prognosis of osteosarcoma patients.
Keywords: miR-27b; osteosarcoma; diagnosis; prognosis; biomarker