文章摘要

HCN1,HCN2在功能性便秘大鼠结肠的分布及其与 Cajal间质细胞的共存关系

作者: 1肖 秋平, 2贾 玉龙, 1陈 云艳, 1耿 学斯
1 厦门市中医院肛肠科,福建 厦门 361009
2 厦门市医药研究所,厦门市天然药物研究与开发重点实验室,福建 厦门 361008
通讯: 耿 学斯 Email: xmgck@sina.com
DOI: 10.3978/j.issn.2095-6959.2017.12.001
基金: 2012年厦门市科技局指导性项目(201209),2016年福建省卫生和计划生育委员会青年科研项目(2016-2-73)。

摘要

目的:观察HCN1,HCN2在功能性便秘(function constipation,FC)大鼠模型结肠上的表达及其与结肠Cajal间质细胞(interstitial cells of Cajal,ICCs)细胞之间的关系,探讨HCN1,HCN2对FC发病的影响。方法:用复方地芬诺酯制造FC大鼠模型,利用免疫组织化学分别对模型组(n=12)、空白组(n=12)大鼠升结肠、横结肠、降结肠黏膜HCN1,HCN2及ICC的表达情况进行检测。结果:模型组和空白组升结肠、横结肠及降结肠均见HCN1,HCN2,c-Kit表达,模型组中HCN1,HCN2,c-Kit表达量明显低于空白组(P<0.05);HCN1表达在FC大鼠结肠中主要分布在结肠环形肌和纵行肌之间的肌ICC-MY,与c-Kit表达分布基本一致;HCN2在FC大鼠升结肠、横结肠及降结肠均有分布与c-Kit荧光度变化趋势基本一致。HCN2阳性神经元与ICCs的突起距离较近,二者未见细胞共存现象。结论:HCN通道作为一种与自主起博活动密切相关的离子通道,可能参与结肠ICCs的自主起博活动。HCN1,HCN2可能参与结肠运动调控,其数量、功能及分布异常可能与FC 发病机制有关。
关键词: 功能性便秘;HCN1;HCN2;结肠Cajal间质细胞

Distribution of HCN1, HCN2 in colon of functional constipation rats and its co-localization with interstitial cells of Cajal

Authors: 1XIAO Qiuping, 2JIA Yulong, 1CHEN Yunyan, 1GENG Xuesi
1 Anorectal Surgery, Xiamen Hospital of Traditional Chinese Medicine, Xiamen Fujian 361009, China
2 Xiamen Institute of Medicine, Xiamen Natural Drug Research and Development Key Laboratory, Xiamen Fujian 361008, China

CorrespondingAuthor:GENG Xuesi
Email: xmgck@sina.com

Foundation: This work was supported by the Plan from Xiamen Science and Technology Program (201209) and the Project from Youth Foundation of Health Department of Fujian Province (2016-2-73), China.

Abstract

Objective: To investigate the distribution of HCN1, HCN2 in colon of functional constipation rats and its co-localization with interstitial cells of Cajal (ICCs) for study on HCN1, HCN2 which play the role of the onset of action in functional constipation. Methods: Twenty-four SD female rats in 12-week were randomized into a normal group (n=12) and a model group (n=12). The immunofluorescence double-labeling stain was used to detect the distribution of HCN1, HCN2, ICC in mucosa of ascending colon, transverse colon and descending colon in functional constipation rats triggered by compound diphenoxylate. Results: HCN1, HCN2 and ICC expressed in mucosa of ascending colon, transverse colon and descending colon tend to be positive after double libeling with c-Kit both in normal and model group. HCN1 expression mainly distributed in ICC-MY between Ring muscle and Longitudinal muscle which was the same with c-Kit expression. HCN2 positive neurons was close to ICCs, which had no co-localization. Conclusion: HCN channel which is closely related with independent blogging activity may participate in colon ICCs independent blogging activity. HCN1, HCN2 may take part in regulation of colon motility. The number, function and the distribution may be related to pathogenesis of functional constipation.
Keywords: functional constipation; HCN1; HCN2; the colon Cajal mesenchymal cells

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