Objective: To discuss and analyze the effect of bundle care nursing measure on alleviating breast cancer chemotherapy related oral mucositis. Methods: Breast cancer patients who accepted chemotherapy and had chemotherapy related oral mucositis in Department of Tumor and Hematology of Yan’an People’s Hospital were selected as the research objects and were divided into an observation group and a control group randomly with the method of random number table. The control group adopted routine oral nursing intervention measures, while the observation group adopted bundle care intervention measures. Scores of oral mucous membrane injury degree, scores of pain in oral cavity, 7-day recovery effect and total healing time of the two groups before intervention (D0) and on the 3rd day (D3), 5th day (D5) and 7th day (D7) after intervention were compared. Results: Scores of pain in oral cavity and scores of oral mucous membrane injury degree of the two groups from D3 to D7 were evidently lower than those on D0, and such scores of the observation group were evidently lower than those of the control group, and the differences were of statistical significance (P<0.05). On the 7th day after intervention, there were 24 recovery cases, 17 effect-showing cases, 10 effective cases and 2 ineffective cases in the observation group; in contrast, there were 11 recovery cases, 14 effect-showing cases, 19 effective cases and 9 ineffective cases in the control group, and the differences were of statistical significance (P<0.05). The oral mucositis healing time of the observation group was (6.63±1.82) days, which was shorter than that of the control group (11.35±2.36) days, and the differences were of statistical significance (P<0.05). Conclusion: Clinical bundle care intervention can effectively accelerate the wound healing of breast cancer chemotherapy related oral mucositis, alleviate pain in oral cavity, and promote rehabilitation.