系统性间变性大细胞淋巴瘤的免疫组织化学特征
| 作者: |
1李鑫静,
1陈宝珍,
1陈燕坪,
1许春伟,
1陈刚,
1郑雄伟
1 福建医科大学附属福建省肿瘤医院病理科，福州 350014 |
| 通讯: |
陈刚
Email: naichengang@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2017.05.017 |
| 基金: | 国家临床重点专科建设项目, 2013 福建省自然科学基金, 2016J01512 福建省卫生厅中青年骨干人才培养资助计划, 2013-ZQN-ZD-7 |
摘要
目的：探讨系统性间变性大细胞淋巴瘤(anaplastic large cell lymphoma，ALCL)的免疫组织化学特征。方法：回顾性分析48例系统性ALCL的免疫组织化学和10例系统性ALCL原位杂交技术检测EBER(EBV-encoded small RNA)的结果。结果：48例系统性ALCL的肿瘤细胞均表达CD30，而间变性淋巴瘤激酶(anaplastic lymphoma kinase，ALK)在41.7%(20/48)的病例阳性，其他指标阳性率为CD2为65.0%(26/40)，CD3为36.2%(17/47)，CD4为72.7%(16/22)，CD5为42.9%(15/35)，CD7为16.7%(5/30)，上皮细胞膜抗原(epithelial membrane antigen，EMA)为65.6%(21/32)，T细胞胞质内抗原(T-cell intracellular antigen-1，TIA-1)为79.2%(19/24)，颗粒酶B(granzyme B-producing Breg，GrB)为70.0%(14/20)。所有病例的B细胞标志(CD20，PAX5，CD79α)均阴性。10例系统性ALCL有2例出现部分肿瘤性大细胞EBER阳性。结论：CD30和ALK是诊断ALCL关键及较为特异的免疫指标；有时出现人类疱疹病毒第四型(Epstein–Barr virus，EBV)感染并不能排除ALCL的诊断。
关键词：
间变性大细胞淋巴瘤
免疫组织化学
原位杂交
人类疱疹病毒第四型感染
Immunohistochemical features of systemic anaplastic large cell lymphoma
CorrespondingAuthor: CHEN Gang Email: naichengang@126.com
DOI: 10.3978/j.issn.2095-6959.2017.05.017
Abstract
Objective: To study the immunohistochemical characteristics of systemic anaplastic large cell lymphoma. Methods: A retrospective analysis of immunophenotype of 48 cases of systemic anaplastic large cell lymphoma and the result of EBV-encoded small RNA (EBER) expression with in situ hybridization of 10 cases was performed. Results: The tumor cells expressed CD30 in 48 cases of ALCL. Anaplastic lymphoma kinase (ALK) was positive in about 41.7% (20/48) cases. The positive rates of other antigens were 65.0% (26/40) of CD2, 36.2% (17/47) of CD3, 72.7% (16/22) of CD4, 42.9% (15/35) of CD5, 16.7% (5/30) of CD7, 65.6% (21/32) of epithelial membrane antigen (EMA), 79.2% (19/24) of TIA-1, and 70.0% (14/20) of granzyme B-producing Breg (GrB). All of the cases were negative for B-cell markers (CD20, PAX-5 and CD79α). Two of 10 cases showed EBER positive in partial tumor cells. Conclusion: CD30 and ALK are the critical and relatively specific markers for ALCL; Epstein–Barr virus (EBV) involvement may not preclude the diagnosis of ALCL in some cases.