Objective: To compare the effect of intubation with the GlideScope video laryngoscope (GSVL) and Macintosh direct laryngoscope in the infants with cleft lip under general anesthesia, and compare their effects on hemodynamics. Methods: We conducted a prospective study on 60 American Society of Anesthesiologists (ASA) I or II infants from 4 to 10 months undergoing cheiloschisis repairing operation. The patients were randomly divided into two groups: using GlideScope video laryngoscopy (group GS) for intubation, and Macintosh Direct Laryngoscopy (group MA) for intubation. Cormack and Lehane grade (C-L grade), glottic exposure time, catheter insertion time, intubation time, success rate of intubation and the change of heart rate during the intubation time were compared between two groups. Intubation complications were classified by oral injury, dental injury, gingival injury, throat soft tissue injury, hoarseness, etc. Results: Compared with MA group, GS group could improve glottis exposure degree (P<0.05), improve the success rate of primary intubation (P<0.05), but prolonged glottis exposure time, catheterization time and intubation time (P<0.05). There was no significant difference in intubation complications between the two groups (P>0.05). The heart rate of the two groups increased rapidly after intubation, and then returned to the level after induction. When exposed glottis, tracheal intubation and immediately after intubation, the heart rate increase in GS group was significantly slower than that in MA group (P<0.05). Conclusion: GSVL can gain better glottis exposure in the condition of assistant absence in cleft lip infants, improve the success rate of primary intubation, but prolong intubation time.